Revision as of 14:42, 18 July 2013

Notes on various bacterial strains:

E. coli:

Strain	Description	Heritage	References	Comments
Dh5a
BW21342
BW25083
BW25113			[BW25113 at Ecoliwiki	Parent strain of the Keio single-deletion mutant collection.
BW21578	rrnBT14 ΔlacZWJ16	BD792 via BW21342
BW22773	lacIq rrnBT14 ΔlacZWJ16 proC::Tn5-132;
BW26434 (CGSC #7658)	Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514
BW27784				Expresses arabinose transporter constitutively.
BW22786				Expresses arabinose transporter constitutively.

Note on LacI satus of BW strains (copied from http://openwetware.org/wiki/E._coli_genotypes):

"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113.

As strains BW27784 and BW22786 were also constructed from BW25113, the former should also be LacI+.

Nomenclature:

Insertion mutation.

Bibliography:

Khlebnikov 2001: http://mic.sgmjournals.org/content/147/12/3241.long

http://web.mit.edu/7.02/pdfs/nomenclature.pdf

CGSC - Coli Genetic Stock Center: http://cgsc.biology.yale.edu/index.php

http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp