User:Jarle Pahr/Vectors: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jarle Pahr (talk | contribs) No edit summary |
Jarle Pahr (talk | contribs) No edit summary |
||
Line 17: | Line 17: | ||
|- | |- | ||
|pKLAC2||9107||Amp,Acetamidase|| || ||Aetamidase expression allows non-antibiotic selection in K. lactis||[https://www.neb.com/products/n3742-pklac2-vector],[https://www.neb.com/~/media/Catalog/All-Products/6F454BB8401043D798342A0719273945/Datacards%20or%20Manuals/N3742Datasheet-Lot0011210.pdf 2] | |pKLAC2||9107||Amp,Acetamidase|| || ||Aetamidase expression allows non-antibiotic selection in K. lactis||[https://www.neb.com/products/n3742-pklac2-vector],[https://www.neb.com/~/media/Catalog/All-Products/6F454BB8401043D798342A0719273945/Datacards%20or%20Manuals/N3742Datasheet-Lot0011210.pdf 2] | ||
|} | |||
'''Origins of replication:''' | |||
{| class="wikitable" border="1" cellpadding="5" cellspacing="0" | |||
|- | |||
! Names!! Copy number !! Regulation !! References | |||
|- | |||
|pBR322|| || || | |||
|- | |||
|pMB1|| || || | |||
|- | |||
|ColE1|| || || | |||
|- | |||
|RK2|| || || | |||
|} | |} |
Revision as of 10:05, 2 June 2013
Notes and links regarding vectors:
AddGene vector database: http://www.addgene.org/vector-database/
Open construct database: http://www.the-odin.com/ocondb/listings.php
Names | Size | Selective markers | Origin of replication | Copy number | Notes | Reference |
---|---|---|---|---|---|---|
pDrive | 3851 | Amp,Kan | pBR322 | ? | PCR Cloning kit vector | |
pBR322 | 4,361bp | Amp,Tet | pMB1 (ColE1 relative) | ? | http://en.wikipedia.org/wiki/PBR322 | |
pUC18 | 2686 | Amp,LacZ | pMB1 (ColE1 relative) | ? | http://www.mun.ca/biology/scarr/Plasmid_pUC18.html | |
pKLAC2 | 9107 | Amp,Acetamidase | Aetamidase expression allows non-antibiotic selection in K. lactis | [1],2 |
Origins of replication:
Names | Copy number | Regulation | References |
---|---|---|---|
pBR322 | |||
pMB1 | |||
ColE1 | |||
RK2 |