User:Jarle Pahr/lablessons: Difference between revisions
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03.02.13: BSA does not affect the activity of enzymes which don't need it for stabilization (http://en.wikipedia.org/wiki/Bovine_serum_albumin) | 03.02.13: BSA does not affect the activity of enzymes which don't need it for stabilization (http://en.wikipedia.org/wiki/Bovine_serum_albumin) | ||
03.02.13: PciI and AgeI are not Dam sensitive. | |||
Revision as of 07:50, 3 February 2013
01.02.13: LA medium does not solidify before it is heated once.
01.02.13: If cap is screwed tightly on, liquid in container may "boil over" when container is opened after autoclaving.
01.02.13: Time for 500 mL LA medium in 1000 mL Schott bottle to cool from ~55 C to a temperature that is not painfull, when the wrist is touched to the bottle (temp when it's OK to add antibiotic): ~40 min
01.02.13: Time for LA plates to solidify: < 20 min
01.02.13: Time for agarose gel to solidify: ?
01.02.13: Commonly used Kan concentration on LA plates: 25-50 ug/mL
02.02:13: If toothpicks are used for inoculation instead of an inoculating needle, there's no danger of inoculating the same sample twice by mistake.
03.02.13: Don't tighten the centrifuge rotor cover too tightly. If it's hard to hopen, a forceps can be used to gain leverage.
03.02.13: BSA does not affect the activity of enzymes which don't need it for stabilization (http://en.wikipedia.org/wiki/Bovine_serum_albumin)
03.02.13: PciI and AgeI are not Dam sensitive.
