User:Jason R. Kelly: Difference between revisions

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(google gadgets are pretty neat)
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tahnks for the tree frog google, you can find other [http://www.google.com/ig/directory?synd=open google gadgets here]
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Revision as of 09:53, 5 October 2006

Bio

Jason Kelly

4th Year Biological Engineering Graduate Student @ MIT in the Endy Lab

S.B. in Biology and Chemical Engineering @ MIT, 2003

Contact

jasonk AT mit DOT edu

Activities

Endy lab fire marshall

OpenWetWare steering committee coordinator - please help out!

Synchronized pipetting

Thesis Project

To date, engineered biological systems have been constructed via a variety of ad hoc approaches. The resulting systems should be thought of as pieces of art. We are interested in exploring how existing forward engineering approaches might be best combined with directed evolution to make routine the construction of engineered biological systems. We have specified a procedure for construction of biological systems via screening of subcomponent libraries and rational re-assembly. We have begun development of tools to enable this approach, including a FACS-based screening system to rapidly measure the input/output function of a genetic circuit. Additionally, we have designed a microfluidic system that enables more sophisticated screening and selection functions. Specifically, a microfluidic chemostat integrated with a cell sorter (i.e., a sort-o-stat). This microscope-based system will enable us to evaluate whether or not more complicated screens and selections will be of practical use in service of evolving engineered biological systems.

Specific Aims

  1. PoPS device screening plasmid
  2. Library-based Construction
  3. Evolutionary Stability

Communications

Current Project Pages

OWW Pages in Progess

BioBricks construction tutorial - a step-by-step tutorial for a single Biobrick assembly.

Microfluidics - a page describing the endy lab microfluidics setup and linking to relevant protocols, etc.

BE Board - homepage for the BE Board

OpenWetWare:Presentations - a presentation for introducing new labs to Openwetware.

ICampus Application for OWW - application for an MIT iCampus grant for OpenWetWare (and a good desciption of the site and future goals)

Science 2.0 - some musings by the OWW community about the internet (Web 2.0) and science

Community Portal v2 - playing around with Community portal organization.

BE Board:Dinner Discussion/Alternate publishing models - discussion for BE retreat

User:Jasonk/sidebar - my sidebar, this is a great feature!

OpenWetWare:Steering committee/NSF BDI Grant - help us write a grant for OWW.

OpenWetWare:Steering committee/Goals

OpenWetWare:Presentations/SciFoo

Jason Kelly/sample wikipedia page for OWW

Flow cytometry

OpenWetWare:Steering Committee v2

edit your sidebar

Glycerols

Papers of Interest

Library-based Construction

Evolutionary Stability

Inhibition of Mutation and Combating of the Evolution of Antibiotic Resistance (Cirz et al)


Random

  • Square bacteria

MIT audio bridge conference calling

Testing this (thanks barry)

<html> <a href="http://clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/Jason_Kelly" id="clustrMapsLink"><img src="http://clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/Jason_Kelly" border=1 alt="Locations of visitors to this page"onError="this.onError=null; this.src='http://www.meetomatic.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com/'"> </a> <script src="http://gmodules.com/ig/ifr?url=http://abowman.googlepages.com/treefrog.xml&synd=open&w=320&h=200&title=__MSG_title__&.lang=de&.country=ALL&border=http%3A%2F%2Fgmodules.com%2Fig%2Fimages%2F&output=js"></script> </html> tahnks for the tree frog google, you can find other google gadgets here