User:Jason R. Kelly: Difference between revisions
No edit summary |
|||
Line 5: | Line 5: | ||
S.B. in Biology and Chemical Engineering @ MIT, 2003 | S.B. in Biology and Chemical Engineering @ MIT, 2003 | ||
==Activities== | ==Activities== | ||
Line 22: | Line 21: | ||
[http://hdl.handle.net/1721.1/21169 Thesis Proposal PDF] | [http://hdl.handle.net/1721.1/21169 Thesis Proposal PDF] | ||
==OWW Pages in Progess== | ==OWW Pages in Progess== | ||
Line 42: | Line 38: | ||
[[Inhibition of Mutation and Combating of the Evolution of Antibiotic Resistance (Cirz et al)]] | [[Inhibition of Mutation and Combating of the Evolution of Antibiotic Resistance (Cirz et al)]] | ||
Revision as of 16:27, 25 October 2005
Bio
Jason Kelly
2nd Year Biological Engineering Graduate Student @ MIT
S.B. in Biology and Chemical Engineering @ MIT, 2003
Activities
BE undergrad curriculum representative
Thesis Project
To date, engineered biological systems have been constructed via a variety of ad hoc approaches. The resulting systems should be thought of as pieces of art. Here, I propose to explore how existing forward engineering approaches might be combined with evolution to make routine the construction of engineered biological systems. I will specify a procedure for construction of biological systems via screening of subcomponent libraries and rational re-assembly. I will develop tools to enable this approach including a high-throughput screening system to measure the input/output function of an arbitrary genetic device. I will apply this approach to construct a collection of ring oscillators and bi-stable switches. Furthermore, I anticipate that performance of these devices will decay over time due to spontaneous errors in replication of the genetic information encoding the systems. As an engineer, I would like to be able to design systems with behavior that is predictable in the face of mutation and selection. I will explore mechanisms for increasing or decreasing the susceptibility of engineered biological systems to loss of function as a result of mutation.
Specific Aims
OWW Pages in Progess
BioBricks construction tutorial - a step-by-step tutorial for a single Biobrick assembly.
Microfluidics - a page describing the endy lab microfluidics setup and linking to relevant protocols, etc.
BE Board - homepage for the BE Board
OWW Presentation - a presentation for introducing new labs to Openwetware.
Papers of Interest
Library-based Construction
Evolutionary Stability
Inhibition of Mutation and Combating of the Evolution of Antibiotic Resistance (Cirz et al)