User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/01: Difference between revisions
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## 177uL 30% H2O2 into 50mL buffer ---> Should be 45mM | ## 177uL 30% H2O2 into 50mL buffer ---> Should be 45mM | ||
## Check this concentration! The molar absorptivity of H2O2 at 240nm is 40,000 M<sup>-1</sup>cm<sup>-1 | ## Check this concentration! The molar absorptivity of H2O2 at 240nm is 40,000 M<sup>-1</sup>cm<sup>-1 | ||
We worked up the data of Trisha's group due to the fact that no other data worked. | |||
The peaked of the concentration for this figure is 390 nm | |||
"Figure 1. Decrease in concentration of luminol during the oxidation of luminol by HRP | "Figure 1. Decrease in concentration of luminol during the oxidation of luminol by HRP | ||
[[Image:Decrease in concentration luminol by oxidation JAvier Vinals.png]] | [[Image:Decrease in concentration luminol by oxidation JAvier Vinals.png]] | ||
We worked up the data of Trisha's group due to the fact that no other data worked. | |||
The peaked of the concentration for this figure is 425 nm | |||
"Figure 2. Increase in concentration of luminol during the oxidation of luminol by HRP" | "Figure 2. Increase in concentration of luminol during the oxidation of luminol by HRP" |
Revision as of 12:30, 8 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry title
ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. DescriptionThree sets of measurements will be performed today.
DataStock Solution
"Figure 1. Decrease in concentration of luminol during the oxidation of luminol by HRP We worked up the data of Trisha's group due to the fact that no other data worked. The peaked of the concentration for this figure is 425 nm "Figure 2. Increase in concentration of luminol during the oxidation of luminol by HRP" |