User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/01: Difference between revisions
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## 177uL 30% H2O2 into 50mL buffer ---> Should be 45mM | ## 177uL 30% H2O2 into 50mL buffer ---> Should be 45mM | ||
## Check this concentration! The molar absorptivity of H2O2 at 240nm is 40,000 M<sup>-1</sup>cm<sup>-1 | ## Check this concentration! The molar absorptivity of H2O2 at 240nm is 40,000 M<sup>-1</sup>cm<sup>-1 | ||
"Figure 1. Decrease in concentration of luminol during the oxidation of luminol by HRP | |||
[[Image:Decrease in concentration luminol by oxidation JAvier Vinals.png]] | |||
Revision as of 12:22, 8 October 2013
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Entry title
ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. DescriptionThree sets of measurements will be performed today.
DataStock Solution
"Figure 1. Decrease in concentration of luminol during the oxidation of luminol by HRP
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