User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/01
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Entry title
ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. DescriptionThree sets of measurements will be performed today.
DataStock Solution
For today's lab, we took UV-Vis spectra of luminol, HRP and H2O2, and we plotted them in the data after their absorbance was corrected. For some reason, the data in the kinetics only work for Trisha's group, and since the next day, the chemiluminescence was not working, we worked in the data from the previous day. "Figure 1. Corrected abosrbance of luminol, HRP, H2O2" Today, we worked up the data of Trisha's group due to the fact that no other data worked. The peaked of the concentration for this figure is 390 nm "Figure 1. Decrease in concentration of luminol during the oxidation of luminol by HRP We worked up the data of Trisha's group due to the fact that no other data worked. The peaked of the concentration for this figure is 425 nm "Figure 2. Increase in concentration of luminol during the oxidation of luminol by HRP" |