User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/16: Difference between revisions
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== | ==Objective== | ||
We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol | |||
==Data== | |||
<u>Stock Solution</u> | |||
# Buffer | |||
## 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM | |||
# Luminol | |||
## Dissolve 12.9mg luminol in 300uL of DMSO | |||
## Add to 50mL buffer ---> 1.46mM | |||
# H2O2 | |||
## 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM | |||
## Check absorption at 250 [http://www.bio.net/mm/methods/1995-January/023533.html source] (ε(250) = 16.69 M<sup>-1</sup>cm<sup>-1</sup>). A = 0.7392. [H2O2] = 44.29mM | |||
We run UV-VIS sample of Citrate and HRP with AUNP, and after analyzed the data correcting the absorbance we observed the following graph. | |||
For citrate the peak was | |||
For HRP the peak was | |||
"Figure 1. Absorbance of Citrate-AUNP and HRP-AUNP" | "Figure 1. Absorbance of Citrate-AUNP and HRP-AUNP" | ||
Revision as of 11:02, 22 October 2013
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ObjectiveWe are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol DataStock Solution
We run UV-VIS sample of Citrate and HRP with AUNP, and after analyzed the data correcting the absorbance we observed the following graph. For citrate the peak was For HRP the peak was "Figure 1. Absorbance of Citrate-AUNP and HRP-AUNP"
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