User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/23: Difference between revisions

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==Objective==
In this class we run a UV vis of every sample we made the day before. After this the data was analyzed, to see the activity of HRP and Pepsin. After making the samples, it was observed that HRP was not active, and no nanoparticles were active. On the other hand, Pepsin was active.  
Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
 
Figure 1. HRP AUnp absorbance vs wavelength
 
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Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.




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In this class we run a UV vis of every sample we made the day before. After this the data was analyzed, to see the activity of HRP and Pepsin. After making the samples, it was observed that HRP was not active, and no nanoparticles were active. On the other hand, Pepsin was active.

Figure 1. HRP AUnp absorbance vs wavelength

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