User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/11/06: Difference between revisions

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==Objective==
==Objective==
Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
After the nanoparticles were taken out of the oven, we run UV-Vis spectra in all the samples, and analyzed the data to see what were the results.


Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.
'Figure 1. Absorption spectra of Myoglobin'
 
[[Image:Spectra Absorption of Myoglobin UV-Vis Javier Vinals.png|500px]]
 
'Figure 2. Absorption spectra of Lysozyme'
 
[[Image:Lysozyme absorption spectra UV-VIs JAvier Vinals.png|500px]]




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Objective

After the nanoparticles were taken out of the oven, we run UV-Vis spectra in all the samples, and analyzed the data to see what were the results.

'Figure 1. Absorption spectra of Myoglobin'

'Figure 2. Absorption spectra of Lysozyme'



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