User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/02/26

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==Objective==
==Objective==
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Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
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Today we'll be recording the results of 30:1 lysozyme AuNPs with variables added before heating at 37 degrees Celsius overnight. Then we will take UV-Vis of the samples with salts added after heating overnight, and we will synthesize 30:1 lysozyme AUNPs, and 60:1,70:1,80:1,90:1,110:1 BSA-AuNPs with MgCl,sub.2 for 4 hours at 80 degress.  
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Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.
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==Procedure==
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===Synthesizing Lysozyme-AuNP and BSA-AuNP===
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* The following figure details how the stock solutions were made as well as the BSA-AuNPs and lysozyme-AuNPs.
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* One tube of each ratio was made for BSA-AuNPs.
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* For lysozyme-AuNPs, 25 tubes were made.  
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* The samples were heated in the oven at 80°C for 4 hours.  
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[[Image:lysbsa.2.26.png|700px|]]
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==Figures==
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===UV-Vis of Heated Samples and Variables (36°C)===
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[[Image:Screen_shot_2014-02-26_at_5.18.42_PM.png]]
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[[Image:Screen_shot_2014-02-26_at_5.18.29_PM.png]]
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[[Image:Screen_shot_2014-02-26_at_5.18.18_PM.png]]
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[[Image:Screen_shot_2014-02-26_at_5.17.57_PM.png]]
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[[Image:Screen_shot_2014-02-26_at_5.17.46_PM.png]]
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[[Image:Screen_shot_2014-02-26_at_5.17.36_PM.png]]
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[[Image:Screen_shot_2014-02-26_at_5.17.23_PM.png]]
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==Notes==
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[[Image:afterheating.png|700px|]]
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These results sum up the detail of the samples as seen below, but being at 37 degrees before observations. After observations, the samples were placed in the fridge.
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Revision as of 19:11, 16 March 2014

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Objective

Today we'll be recording the results of 30:1 lysozyme AuNPs with variables added before heating at 37 degrees Celsius overnight. Then we will take UV-Vis of the samples with salts added after heating overnight, and we will synthesize 30:1 lysozyme AUNPs, and 60:1,70:1,80:1,90:1,110:1 BSA-AuNPs with MgCl,sub.2 for 4 hours at 80 degress.

Procedure

Synthesizing Lysozyme-AuNP and BSA-AuNP

  • The following figure details how the stock solutions were made as well as the BSA-AuNPs and lysozyme-AuNPs.
  • One tube of each ratio was made for BSA-AuNPs.
  • For lysozyme-AuNPs, 25 tubes were made.
  • The samples were heated in the oven at 80°C for 4 hours.

Figures

UV-Vis of Heated Samples and Variables (36°C)

Image:Screen_shot_2014-02-26_at_5.18.42_PM.png Image:Screen_shot_2014-02-26_at_5.18.29_PM.png Image:Screen_shot_2014-02-26_at_5.18.18_PM.png Image:Screen_shot_2014-02-26_at_5.17.57_PM.png Image:Screen_shot_2014-02-26_at_5.17.46_PM.png Image:Screen_shot_2014-02-26_at_5.17.36_PM.png Image:Screen_shot_2014-02-26_at_5.17.23_PM.png

Notes

These results sum up the detail of the samples as seen below, but being at 37 degrees before observations. After observations, the samples were placed in the fridge.


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