User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/03/04: Difference between revisions

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==Objective==
==Objective==
Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
Today we'll be adding the following variables to the 30:1 lysozyme AuNPs synthesized without salts: NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub>, 2,2 bipyridine, MES, citric acid. Then we will run UV-Vis of 30:1 lysozyme AuNPs with variables added after they were cooled to 4 degrees celsius over the weekend.


Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.
==Procedure==
===Preparing Samples for Atomic Absorption===
* To the 30:1 lysozyme-AuNP samples synthesized with salts, 2.71 mL of the variables were added. Three concentrations for each were tested: 100 mM, 10 mM, and 1 mM.  
::* For 2,2 bipyridine, the concentrations were 9 mM, 0.9 mM, and 0.09 mM.
* The samples were left to sit at room temperature overnight.
 
==Figures==
 
*Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 4 Degrees. 
[[Image:Screen_Shot_2014-03-13_at_2.35.34_PM.png]]
[[Image:Screen_Shot_2014-03-13_at_2.35.49_PM.png]]
[[Image:Screen_Shot_2014-03-13_at_2.36.04_PM.png]]
[[Image:Screen_Shot_2014-03-13_at_2.36.19_PM.png]]
[[Image:Screen_Shot_2014-03-13_at_2.36.33_PM.png]]
[[Image:Screen_Shot_2014-03-13_at_2.36.47_PM.png]]
[[Image:Screen_Shot_2014-03-13_at_2.37.07_PM.png]]
 
==Notes==
===Observations of 30:1 lysozyme-AuNP Samples Cooled to 4°C With Variables Added After Synthesizing===
[[Image:3.4.observations.png|800px|]]




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Objective

Today we'll be adding the following variables to the 30:1 lysozyme AuNPs synthesized without salts: NaCl, KCl, MgCl2, CaCl2, 2,2 bipyridine, MES, citric acid. Then we will run UV-Vis of 30:1 lysozyme AuNPs with variables added after they were cooled to 4 degrees celsius over the weekend.

Procedure

Preparing Samples for Atomic Absorption

  • To the 30:1 lysozyme-AuNP samples synthesized with salts, 2.71 mL of the variables were added. Three concentrations for each were tested: 100 mM, 10 mM, and 1 mM.
  • For 2,2 bipyridine, the concentrations were 9 mM, 0.9 mM, and 0.09 mM.
  • The samples were left to sit at room temperature overnight.

Figures

  • Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 4 Degrees.

Notes

Observations of 30:1 lysozyme-AuNP Samples Cooled to 4°C With Variables Added After Synthesizing



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