User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/03/05: Difference between revisions
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==Objective== | ==Objective== | ||
Today we'll be | Today we'll be calculating the equilibrium constants for heated 30:1 lysozyme AuNPs with variables added after baking, and room temperature 30:1 lysozyme AuNPs. Then we will make standards with the amount of gold per 5mL tube to run AA and UV-Vis. Then we will centrifuge the samples from yesterday, and run AA and UV-Vis on centrifuged samples. | ||
==Procedure== | |||
===Atomic Absorption=== | |||
'''Creating the Gold Stock Solutions''' | |||
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. | |||
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
# A sixth stock solution of gold was created with 448 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4552 μL of distilled water to a Falcon tube. This resulting concentration represents the amount of gold in a 5 mL sample of lysozyme-AuNP. | |||
'''Atomic Absorption Samples''' | |||
The following samples were run on the AA: | |||
* 30:1 lysozyme-AuNP with NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub>, 2,2 bipyridine, MES, citric acid | |||
* 60:1, 70:1, 80:1, 90:1, 100:1 BSA-AuNP with MgCl<sub>2</sub> | |||
* Gold solution in 5 mL sample of lysozyme-AuNP | |||
* Control of 30:1 lysozyme-AuNP without any added variable | |||
Revision as of 16:21, 16 March 2014
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Entry title
ObjectiveToday we'll be calculating the equilibrium constants for heated 30:1 lysozyme AuNPs with variables added after baking, and room temperature 30:1 lysozyme AuNPs. Then we will make standards with the amount of gold per 5mL tube to run AA and UV-Vis. Then we will centrifuge the samples from yesterday, and run AA and UV-Vis on centrifuged samples. ProcedureAtomic AbsorptionCreating the Gold Stock Solutions
Atomic Absorption Samples The following samples were run on the AA:
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