User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/03/25: Difference between revisions
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==Objective== | ==Objective== | ||
Today we'll be | Today we'll be adding three different concentrations of 30,60, and 90 mM to the 30:1 lysozyme AuNP solutions at three temperatures (4, 22, 37 degrees). Then we will synthesize an additional tube of 30:1 lysozyme AuNP to use as a standard future testing. | ||
==Procedure== | |||
''Preparing Samples for Addition of Variables'' | |||
* Pipette 5 mL aliquots into a Falcon tube from the 15 mL samples. | |||
''Dilution of Variables'' | |||
* The following table shows how we diluted the stock concentrations of various variables. | |||
* The first graph shows the dilution to 30 mM, the second graph shows 60 mM, and the third graph shows 90 mM. | |||
* The concentrations of 2,2 bipyridine used are 0.0002316 M, 0.0004544 M, and 0.0006772 M from a stock concentration of 0.0099 M. | |||
[[Image:3.25.dilution.jac.png|950px|]] | |||
''Adding Variables to 30:1 Lysozyme-AuNP Solutions'' | |||
# Add 2.5 mL of each variable to each tube. | |||
# Place 21 tubes in the oven at 36°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub>, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine. | |||
# Place 21 tubes in the freezer at 4°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub>, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine. | |||
# Place 21 tubes on the bench at 22°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub>, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine. | |||
''Synthesizing 30:1 Lysozyme-AuNP'' | |||
* The following figure describes how we made one tube of 30:1 lysozyme-AuNPs. | |||
* The sample was heated at 80°C for 4 hours. | |||
[[Image:3.25.one.tube.png|750px|]] | |||
Revision as of 09:44, 26 March 2014
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Entry title
ObjectiveToday we'll be adding three different concentrations of 30,60, and 90 mM to the 30:1 lysozyme AuNP solutions at three temperatures (4, 22, 37 degrees). Then we will synthesize an additional tube of 30:1 lysozyme AuNP to use as a standard future testing. ProcedurePreparing Samples for Addition of Variables
Dilution of Variables
|