User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/04/01: Difference between revisions
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==Objective== | ==Objective== | ||
* Run UV-Vis and AA on cooled 30:1 lysozyme-AuNP samples from 3/25/14. | |||
* Run conductivity on room temperature 30:1 lysozyme-AuNP samples from 3/25/14. | |||
==Procedure== | |||
===Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14=== | |||
1. Add 2.25 mL of each variable to 4.5 mL of sample. | |||
:* The variables used were: | |||
::* 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | |||
::* 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | |||
::* 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | |||
2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C. | |||
===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature=== | |||
[[Image:4.1.conductivity.jac.png|800px|]] | |||
===Atomic Absorption Preparation=== | |||
'''Creating the Gold Stock Solutions''' | |||
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. | |||
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
'''Atomic Absorption Samples''' | |||
Solutions with the following Au:lysozyme ratio at room temperature were run on the AA: | |||
* 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | |||
===Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples=== | |||
# Add 2.841 g of sodium citrate filled with deionized water to 10 mL in a 15 mL Falcon tube. | |||
# Using serial dilutions, 1 mM, 10 mM, and 100 mM solutions were created. | |||
# Take 1 mL aliquots from 30:1 lysozyme-AuNP samples and add 0.5 mL of each concentration of sodium citrate. | |||
==Figures== | |||
[[Image: | [[Image:4.1.jac.aa.png|800px|]] | ||
Revision as of 08:20, 7 April 2014
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Entry title
Objective
ProcedureAdding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/141. Add 2.25 mL of each variable to 4.5 mL of sample.
2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.
Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature
Atomic Absorption PreparationCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:
Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples
Figures
|