User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/04/01

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2014/04/01 Entry for User:Javier_Vinals_Camallonga/Notebook/Javier_Vinals_notebook)
(Objective)
Line 10: Line 10:
==Objective==
==Objective==
-
Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
+
• Synthesize 30:1 lysozyme-AuNP.
 +
 +
 
 +
 
 +
==Procedure==
 +
'''Creating Stock Solutions of Gold and Lysozyme'''
 +
* As seen below, each stock solution was created following the calculations in the table.
 +
* Each solution was created using a volumetric flask and deionized water.
 +
 
 +
[[Image:3.19.data.2.png|800px|]]
 +
 
 +
 
 +
'''Synthesizing Lysozyme-AuNPs'''
 +
* As seen below, each solutions was created using the following calculations.
 +
* We made 21 tubes total.  
 +
 
 +
[[Image:3.19.data.1.png|650px|]]
-
Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.
 

Revision as of 12:44, 2 April 2014

Project name Main project page
Previous entry      Next entry

Entry title

  • Insert content here...

Objective

• Synthesize 30:1 lysozyme-AuNP. •


Procedure

Creating Stock Solutions of Gold and Lysozyme

  • As seen below, each stock solution was created following the calculations in the table.
  • Each solution was created using a volumetric flask and deionized water.


Synthesizing Lysozyme-AuNPs

  • As seen below, each solutions was created using the following calculations.
  • We made 21 tubes total.



Personal tools