User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/04/01

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(Objective)
Current revision (10:20, 7 April 2014) (view source)
(Procedure)
 
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==Procedure==
==Procedure==
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'''Creating Stock Solutions of Gold and Lysozyme'''
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===Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14===
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* As seen below, each stock solution was created following the calculations in the table.
+
1.  Add 2.25 mL of each variable to 4.5 mL of sample.
-
* Each solution was created using a volumetric flask and deionized water.  
+
:* The variables used were:
 +
::* 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
 +
::* 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
 +
::* 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
 +
2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.
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[[Image:3.19.data.2.png|800px|]]
 
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'''Synthesizing Lysozyme-AuNPs'''
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===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature===
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* As seen below, each solutions was created using the following calculations.
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[[Image:4.1.conductivity.jac.png|800px|]]
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* We made 21 tubes total.  
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[[Image:3.19.data.1.png|650px|]]
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 +
 
 +
===Atomic Absorption Preparation===
 +
 
 +
'''Creating the Gold Stock Solutions'''
 +
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
 +
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
 +
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
 +
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
 +
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
 +
 
 +
 
 +
 
 +
'''Atomic Absorption Samples'''
 +
 
 +
Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:
 +
* 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
 +
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
 +
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
 +
 
 +
 
 +
 
 +
 
 +
===Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples===
 +
# Add 2.841 g of sodium citrate filled with deionized water to 10 mL in a 15 mL Falcon tube.
 +
# Using serial dilutions, 1 mM, 10 mM, and 100 mM solutions were created.
 +
# Take 1 mL aliquots from 30:1 lysozyme-AuNP samples and add 0.5 mL of each concentration of sodium citrate.
 +
 
 +
==Figures==
 +
 
 +
[[Image:4.1.jac.aa.png|800px|]]

Current revision

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Entry title

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Objective

  • Run UV-Vis and AA on cooled 30:1 lysozyme-AuNP samples from 3/25/14.
  • Run conductivity on room temperature 30:1 lysozyme-AuNP samples from 3/25/14.

Procedure

Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14

1. Add 2.25 mL of each variable to 4.5 mL of sample.

  • The variables used were:
  • 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.


Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature


Atomic Absorption Preparation

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)



Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples

  1. Add 2.841 g of sodium citrate filled with deionized water to 10 mL in a 15 mL Falcon tube.
  2. Using serial dilutions, 1 mM, 10 mM, and 100 mM solutions were created.
  3. Take 1 mL aliquots from 30:1 lysozyme-AuNP samples and add 0.5 mL of each concentration of sodium citrate.

Figures



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