User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/04/15

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(Autocreate 2014/04/15 Entry for User:Javier_Vinals_Camallonga/Notebook/Javier_Vinals_notebook)
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==Objective==
==Objective==
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Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
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* Take conductivity measurements on 36°C solutions from 3/26/14.  
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* Finish UV-Vis on solutions at 36°C from 3/26/14.
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==Procedure==
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===Conductivity Measurements of 30:1 Lysozyme-AuNP at 36°C===
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*Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated.
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[[Image:4.15.conductiv.png|800px|]]
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*Note that MES and 2,2 Bipyridine have reduced conductivity values. This is because other variables are ions, thus further increase this measurement. This may overwhelm observed effects.  
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Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.
 

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Objective

  • Take conductivity measurements on 36°C solutions from 3/26/14.
  • Finish UV-Vis on solutions at 36°C from 3/26/14.

Procedure

Conductivity Measurements of 30:1 Lysozyme-AuNP at 36°C

  • Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated.

  • Note that MES and 2,2 Bipyridine have reduced conductivity values. This is because other variables are ions, thus further increase this measurement. This may overwhelm observed effects.



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