User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/04/16: Difference between revisions

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==Objective==
==Objective==
Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
* Take conductivity measurements on five different concentrations of gold solutions at room temperature.
*Start trying to work out equilibrium constants for all recent UV-Vis, AA, and conductivity samples.
* Learn how to calculate equilibrium constants from Dr.Hartings!!
==Procedure==
 
===Conductivity Measurements of Gold Standard Solutions at 18°C===
[[Image:conduct.4.16.14.png|600px|]]
*These measurements were taken in order to get controls to compare to when trying to put together equilibrium constants.
 
==Synthesis of three tubes of lyso 30:1 AuNP's==
*Due to wonky AA results for our control sample of normal lysozyme 30:1 AuNP's we decided to synthesize three tubes of control sample and take AA at each of our respective temperatures. This is to guarantee that we have good controls to compare to for data analysis. Samples were synthesized following the same procedure represented in previous days.


Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.




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Entry title

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Objective

  • Take conductivity measurements on five different concentrations of gold solutions at room temperature.
  • Start trying to work out equilibrium constants for all recent UV-Vis, AA, and conductivity samples.
  • Learn how to calculate equilibrium constants from Dr.Hartings!!

Procedure

Conductivity Measurements of Gold Standard Solutions at 18°C

  • These measurements were taken in order to get controls to compare to when trying to put together equilibrium constants.

Synthesis of three tubes of lyso 30:1 AuNP's

  • Due to wonky AA results for our control sample of normal lysozyme 30:1 AuNP's we decided to synthesize three tubes of control sample and take AA at each of our respective temperatures. This is to guarantee that we have good controls to compare to for data analysis. Samples were synthesized following the same procedure represented in previous days.



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