User:JeffreyLau/Notebook/2006-6-20

In the morning, Hetmann and I miniprepped and digested (with Xba and Pst1) our two R0010 + E0241 cultures from yesterday. We also made glycerol stocks of 100 µL of each culture.

After lunch, several of us (David, Tiffany, Matt, Valerie, Katie, Lewis, and I, IIRC) had a meeting with Shawn to discuss designs for our DNA nanostructure. We settled on a simple honeycomb cylinder for now.

Note: DNA width 2nm, helical turn 3.4nm (10.5 bp). DNA gets floppy when longer than 50nm (~150bp)

Afterwards, we made e-gels of our digests. Mine didn't turn out so well (lanes 8 and 9):

I sepnt the rest of the day designing nanostructures with David, Tiffany, Matt, Katie, and Lewis. We made two designs based on a honeycomb cylinder: a large cylinder with two single-ply lids and a smaller cylinder with two double-ply lids. Our figures are written on the whiteboard in the 5th floor breakroom; I'll write them down if no one else recorded them.