User:Jessho: Difference between revisions
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UROP, June 08 - Present, Lauffenburger Lab | UROP, June 08 - Present, Lauffenburger Lab | ||
=== | ===SAGA-Related=== | ||
My Favorite Protein: YJR097W | |||
<u>Primer Design</u> | |||
Purpose: To design a primer to amplify a TAP TRP1 fusion gene. After PCR, the fusion gene will have additional elements, i.e. homologous regions of the S. cerivisiae gene YGR097W flanking the TAP TRP1 gene, to enable C-terminus modification of YGR097W via homologous recombination. | |||
Forward: | |||
5’- AAGGTTAATTTTGACATCGAGGAAGAGCAAGAAGGACAA TCC ATG GAA AAG AGA AGA TG - 3' | |||
Backward: | |||
5'- AGATTTATCTGATATGCTCAATTTCCCCTCCCATTTTCA GAC TCA CTA TAG GGC GA - 3' | |||
Revision as of 12:33, 8 October 2008
Jessica Ho
Last Name
Ho
First Name
Jessica
Preferred name
Jess
Course/Minor
20 and 8B. No minor, yet.
Year of Graduation
2010
Telephone #
(703) 623-2414
jessho AT mit DOT edu
Have you taken or are you taking...
Completed 7.05/5.07 (Biochemistry)
Enrolled 7.06 (Cell Biology)
No 7.02 (General Biology Lab)
No 5.310 (General Chemistry Lab)
Do you have any experience culturing cells (mammalian, yeast or microbial)?
Yes
Do you have any experience in molecular biology (electrophoresis, PCR, etc)?
Yes
Please briefly describe any previous laboratory experience
Undergrad team member, MIT iGEM 2007 team UROP, June 08 - Present, Lauffenburger Lab
SAGA-Related
My Favorite Protein: YJR097W
Primer Design
Purpose: To design a primer to amplify a TAP TRP1 fusion gene. After PCR, the fusion gene will have additional elements, i.e. homologous regions of the S. cerivisiae gene YGR097W flanking the TAP TRP1 gene, to enable C-terminus modification of YGR097W via homologous recombination.
Forward: 5’- AAGGTTAATTTTGACATCGAGGAAGAGCAAGAAGGACAA TCC ATG GAA AAG AGA AGA TG - 3'
Backward: 5'- AGATTTATCTGATATGCTCAATTTCCCCTCCCATTTTCA GAC TCA CTA TAG GGC GA - 3'
