User:Jessica Karen Wong/Design: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→I2055) |
(→I2055) |
||
(7 intermediate revisions by one other user not shown) | |||
Line 25: | Line 25: | ||
==E0240== | ==E0240== | ||
*Version 1 (E/S) | *Version 1 (E/S) | ||
**___E | **___E CCDB S___RBS GFP T | ||
**Cut promoter E/S and insert | **Cut promoter E/S and insert | ||
**Can take part back out if necessary | **Can take part back out if necessary | ||
Line 49: | Line 49: | ||
**Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation | **Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation | ||
**Real condition spacing | **Real condition spacing | ||
*Both versions should be on both 3K3 and 1AK3 | |||
*Theoretically I2056 should be the same w/ RFP | |||
[[Image:I2055-design.JPG|thumb|left|Diagram]] |
Latest revision as of 23:55, 14 August 2007
Constructing an output measurement kit derived from <BBpart> T9002</BBpart> consisting of
- an inducible promoter <BBpart> F2620</BBpart> which produces POPs in the presence of AHL
- a Biobrick site
- The ribosome binding site <BBpart> B0032</BBpart>
- The Green Fluorescent Protein <BBpart> E0040</BBpart>
- The terminator <BBpart> B0015</BBpart>
Assisting in the construction of
- Promoter tester derived from <BBpart> E0240</BBpart>
- RBS tester derived from <BBpart> I2055</BBpart>
Building devices containing RFP <BBpart> E1011</BBpart> and LacZ <BBpart> I0054</BBpart>
- Similar to <BBpart> E0240</BBpart> containing <BBpart> B0032</BBpart> and <BBpart> J04650</BBpart>
- Similar to <BBpart> I2055</BBpart> containing <BBpart> R0040</BBpart> and <BBpart> J04650</BBpart>
T90002
F2620________E X P1010 S P________E0040
Should be on both 3K3 and 1AK3
E0240
- Version 1 (E/S)
- ___E CCDB S___RBS GFP T
- Cut promoter E/S and insert
- Can take part back out if necessary
- Version 2
- ___E CCDB X___E0240
- Cut Promoter E/S to insert
- Forms mixed site so can't remove, real conditions
- Both versions should be on both 3K3 and 1AK3
- I2057 is the exact same but w/ RFP (both versions)
I2055
- Versions 1 (E/X)
- __R0040___E CCDB X____GFP T
- To insert RBS cut E/S, forms a mixed Spe/Xba site
- Has too big spacing (full BB prefix) b/t promoter and RBS
- Easier for testers to use
- Version 2 (S/X)
- __R0040___S CCDB X____GFP T
- Will get 2 spe/xba mixed sites, can't get RBS out
- Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
- Real condition spacing
- Both versions should be on both 3K3 and 1AK3
- Theoretically I2056 should be the same w/ RFP