User:Jessica Karen Wong/Progress: Difference between revisions
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(→E0240) |
(→I2057) |
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*E/S sealed w/ Not1 is seq verified 267-end, waiting on redo for beginning | *E/S sealed w/ Not1 is seq verified 267-end, waiting on redo for beginning | ||
*E/X sealed w/ Not1 is seq verified 1-769, waiting on redo for end | *E/X sealed w/ Not1 is seq verified 1-769, waiting on redo for end | ||
Ligated J116, J100, and R0040 into EX version - waiting on seq | |||
==I2055== | ==I2055== |
Revision as of 13:04, 8 August 2007
T9002
Output Measurement Kit (for inverters, terminators, etc) Using GFP
<BBpart> T9002</BBpart>
- F2620 has Mfe/BB prefix tails
- Got only 1 right size colony from 3K3 ligation/transformation
- Waiting on seq verification
- E0240 has been PCR'ed with tails, looks the right size
- Digesting E0240 S/N to ligate into F2620
E0240
Promoter Tester with GFP
<BBpart> E0240</BBpart>
- Part is built
- PCRed with Mfe1 and Spe1 tails
- Successfully ligated into 3K3 - Sequence verifies that it's scarred
- Inserted a BB site between plasmid and RBS
- Made both E/S and E/X versions
- Has a 1bp mutation around 700
- Did mutagenesis and got a good number of colonies
- Sent in right size colonies for seq
I2057
Promoter tester with RFP
<BBpart> I2057</BBpart>
- Part is built (sequence verified)
- PCR-ed with Mfe1 and Nsi1
- Ligated in 3K3 (seq verified scarred)
- Inserted a BB site b/t plasmid and RBS
- Created both E/X and E/S versions
- E/S sealed w/ Not1 is seq verified 267-end, waiting on redo for beginning
- E/X sealed w/ Not1 is seq verified 1-769, waiting on redo for end
Ligated J116, J100, and R0040 into EX version - waiting on seq
I2055
RBS tester with GFP
<BBpart> I2055</BBpart>
- Most likely built and scarred
- waiting on redo of seq to make sure middle (bp 95-208) is ok
- Inserted a BB site b/t Promoter and GFP
- Made both E/X and S/X forms
- Digesting with each E/X, S/X, and Not1
- Attempting to seal w/ Not1 and get colonies to sequence
I2056
RBS tester with RFP
<BBpart> I2056</BBpart>
- Formerly called blue
- Part is most likely built and scarred
- Waiting on redo of seq to make sure front is scarred
- Inserted a BB site b/t promoter and RFP
- Created both E/X and S/X
- Digested with each E/X, S/X, and Not1
- Attempting to seal w/ Not1 and get colonies to sequence