User:Jessica Perez/CloningWorkflow

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==Source of DNA==
==Source of DNA==
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{ "class": "go.TreeModel",
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<html>
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  "nodeDataArray": [
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<canvas id="myCanvas" width="1000" height="1000"
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{"key":"1", "name":"Corrado 'Junior' Soprano", "title":"The Boss"},
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style="border:12px solid #000000;">
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{"key":"2", "parent":"1", "name":"Tony Soprano", "title":"Underboss"},
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</canvas>
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{"key":"3", "parent":"1", "name":"Herman 'Hesh' Rabkin", "title":"Advisor"},
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<script>
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{"key":"4", "parent":"2", "name":"Paulie Walnuts", "title":"Capo"},
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{"key":"5", "parent":"2", "name":"Ralph Cifaretto", "title":"Capo MIA"},
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{"key":"6", "parent":"2", "name":"Silvio Dante", "title":"Consigliere"},
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var c=document.getElementById("myCanvas");
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{"key":"7", "parent":"2", "name":"Bobby Baccilien", "title":"Capo"},
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var ctx=c.getContext("2d");
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{"key":"8", "parent":"4", "name":"Sal Bonpensiero", "title":"MIA"},
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ctx.fillStyle="#FFFFFF";
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{"key":"9", "parent":"4", "name":"Christopher Moltisanti", "title":"Made Man"},
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ctx.fillRect(400,10,200,75);
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{"key":"10", "parent":"4", "name":"Furio Giunta", "title":"Muscle"},
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{"key":"11", "parent":"4", "name":"Patsy Parisi", "title":"Accountant"}
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]
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}
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var c=document.getElementById("myCanvas");
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var ctx=c.getContext("2d");
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ctx.beginPath();
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ctx.arc(200,200,100,0,2*Math.PI);
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ctx.stroke();
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var c=document.getElementById("myCanvas");
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var ctx=c.getContext("2d");
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ctx.fillStyle="#FF0000";
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ctx.fillRect(200,400,150,75);
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ctx.font="20px Georgia";
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ctx.fillText("Hello World!",10,50);
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</script>
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</html>

Current revision

Planning

Before you start any cloning in the lab you first do the following:

  1. Decide what parts you need
  2. Design primers
  3. Draw up your expected plasmid. This can be done in Word or freely avalible DNA editor software such as APE .

Source of DNA

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