User:Jessica Perez/CloningWorkflow: Difference between revisions

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==Source of DNA==
==Source of DNA==
{ "class": "go.TreeModel",
<html>
  "nodeDataArray": [
<canvas id="myCanvas" width="1000" height="1000"
{"key":"1", "name":"Corrado 'Junior' Soprano", "title":"The Boss"},
style="border:12px solid #000000;">
{"key":"2", "parent":"1", "name":"Tony Soprano", "title":"Underboss"},
</canvas>
{"key":"3", "parent":"1", "name":"Herman 'Hesh' Rabkin", "title":"Advisor"},
<script>
{"key":"4", "parent":"2", "name":"Paulie Walnuts", "title":"Capo"},
 
{"key":"5", "parent":"2", "name":"Ralph Cifaretto", "title":"Capo MIA"},
 
{"key":"6", "parent":"2", "name":"Silvio Dante", "title":"Consigliere"},
var c=document.getElementById("myCanvas");
{"key":"7", "parent":"2", "name":"Bobby Baccilien", "title":"Capo"},
var ctx=c.getContext("2d");
{"key":"8", "parent":"4", "name":"Sal Bonpensiero", "title":"MIA"},
ctx.fillStyle="#FFFFFF";
{"key":"9", "parent":"4", "name":"Christopher Moltisanti", "title":"Made Man"},
ctx.fillRect(400,10,200,75);
{"key":"10", "parent":"4", "name":"Furio Giunta", "title":"Muscle"},
 
{"key":"11", "parent":"4", "name":"Patsy Parisi", "title":"Accountant"}
 
]
 
}
var c=document.getElementById("myCanvas");
var ctx=c.getContext("2d");
ctx.beginPath();
ctx.arc(200,200,100,0,2*Math.PI);
ctx.stroke();
 
var c=document.getElementById("myCanvas");
var ctx=c.getContext("2d");
ctx.fillStyle="#FF0000";
ctx.fillRect(200,400,150,75);
 
ctx.font="20px Georgia";
ctx.fillText("Hello World!",10,50);
 
 
 
</script>
</html>

Latest revision as of 15:36, 3 August 2013

Planning

Before you start any cloning in the lab you first do the following:

  1. Decide what parts you need
  2. Design primers
  3. Draw up your expected plasmid. This can be done in Word or freely avalible DNA editor software such as <html>

<body> <a href="http://biologylabs.utah.edu/jorgensen/wayned/ape/"> APE</a> </body> </html>.

Source of DNA

<html> <canvas id="myCanvas" width="1000" height="1000" style="border:12px solid #000000;"> </canvas> <script>


var c=document.getElementById("myCanvas"); var ctx=c.getContext("2d"); ctx.fillStyle="#FFFFFF"; ctx.fillRect(400,10,200,75);


var c=document.getElementById("myCanvas"); var ctx=c.getContext("2d"); ctx.beginPath(); ctx.arc(200,200,100,0,2*Math.PI); ctx.stroke();

var c=document.getElementById("myCanvas"); var ctx=c.getContext("2d"); ctx.fillStyle="#FF0000"; ctx.fillRect(200,400,150,75);

ctx.font="20px Georgia"; ctx.fillText("Hello World!",10,50);


</script> </html>

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