User:Jessica Perez/CloningWorkflow

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==Source of DNA==
==Source of DNA==
 +
{ "class": "go.TreeModel",
 +
  "nodeDataArray": [
 +
{"key":"1", "name":"Corrado 'Junior' Soprano", "title":"The Boss"},
 +
{"key":"2", "parent":"1", "name":"Tony Soprano", "title":"Underboss"},
 +
{"key":"3", "parent":"1", "name":"Herman 'Hesh' Rabkin", "title":"Advisor"},
 +
{"key":"4", "parent":"2", "name":"Paulie Walnuts", "title":"Capo"},
 +
{"key":"5", "parent":"2", "name":"Ralph Cifaretto", "title":"Capo MIA"},
 +
{"key":"6", "parent":"2", "name":"Silvio Dante", "title":"Consigliere"},
 +
{"key":"7", "parent":"2", "name":"Bobby Baccilien", "title":"Capo"},
 +
{"key":"8", "parent":"4", "name":"Sal Bonpensiero", "title":"MIA"},
 +
{"key":"9", "parent":"4", "name":"Christopher Moltisanti", "title":"Made Man"},
 +
{"key":"10", "parent":"4", "name":"Furio Giunta", "title":"Muscle"},
 +
{"key":"11", "parent":"4", "name":"Patsy Parisi", "title":"Accountant"}
 +
]
 +
}

Revision as of 17:21, 3 August 2013

Planning

Before you start any cloning in the lab you first do the following:

  1. Decide what parts you need
  2. Design primers
  3. Draw up your expected plasmid. This can be done in Word or freely avalible DNA editor software such as APE .

Source of DNA

{ "class": "go.TreeModel",

 "nodeDataArray": [

{"key":"1", "name":"Corrado 'Junior' Soprano", "title":"The Boss"}, {"key":"2", "parent":"1", "name":"Tony Soprano", "title":"Underboss"}, {"key":"3", "parent":"1", "name":"Herman 'Hesh' Rabkin", "title":"Advisor"}, {"key":"4", "parent":"2", "name":"Paulie Walnuts", "title":"Capo"}, {"key":"5", "parent":"2", "name":"Ralph Cifaretto", "title":"Capo MIA"}, {"key":"6", "parent":"2", "name":"Silvio Dante", "title":"Consigliere"}, {"key":"7", "parent":"2", "name":"Bobby Baccilien", "title":"Capo"}, {"key":"8", "parent":"4", "name":"Sal Bonpensiero", "title":"MIA"}, {"key":"9", "parent":"4", "name":"Christopher Moltisanti", "title":"Made Man"}, {"key":"10", "parent":"4", "name":"Furio Giunta", "title":"Muscle"}, {"key":"11", "parent":"4", "name":"Patsy Parisi", "title":"Accountant"}

]

}

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