User:Jessica Perez/CloningWorkflow
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Planning
Before you start any cloning in the lab you first do the following:
- Decide what parts you need
- Design primers
- Draw up your expected plasmid. This can be done in Word or freely avalible DNA editor software such as <html>
<body> <a href="http://biologylabs.utah.edu/jorgensen/wayned/ape/"> APE</a> </body> </html>.
Source of DNA
function init() {
if (window.goSamples) goSamples(); // init for these samples -- you don't need to call this
// Note that we do not use $ here as an alias for go.GraphObject.make because we are using $ for jQuery var GO = go.GraphObject.make; // for conciseness in defining templates
myDiagram = new go.Diagram("myDiagram"); // must name or refer to the DIV HTML element
// define several shared Brushes var grad1 = GO(go.Brush, go.Brush.Linear, { 0: "rgb(115,220,241)", 0.5: "rgb(105,210,231)", 1: "rgb(75,180,201)" }); var brush1 = "rgb(65,180,181)";
var grad2 = GO(go.Brush, go.Brush.Linear, { 0: "rgb(177,229,226)", 0.5: "rgb(167,219,216)", 1: "rgb(137,189,186)" }); var brush2 = "rgb(127,179,176)";
var grad3 = GO(go.Brush, go.Brush.Linear, { 0: "rgb(234,238,214)", 0.5: "rgb(224,228,204)", 1: "rgb(194,198,174)" }); var brush3 = "rgb(184,188,164)";
var grad4 = GO(go.Brush, go.Brush.Linear, { 0: "rgb(253,164,58)", 0.5: "rgb(243,134,48)", 1: "rgb(213,104,18)" }); var brush4 = "rgb(203,84,08)";
myDiagram.nodeTemplateMap.add("", // default GO(go.Node, go.Panel.Auto, { locationSpot: go.Spot.Center }, new go.Binding("location", "loc", go.Point.parse).makeTwoWay(go.Point.stringify), GO(go.Shape, "Ellipse", { strokeWidth: 2, fill: grad1, name: "SHAPE" }, new go.Binding("figure", "figure"), new go.Binding("fill", "fill"), new go.Binding("stroke", "stroke") ), GO(go.TextBlock, { margin: 5, maxSize: new go.Size(200, NaN), wrap: go.TextBlock.WrapFit, textAlign: "center", editable: true, font: "bold 9pt Helvetica, Arial, sans-serif", name: "TEXT" }, new go.Binding("text", "text").makeTwoWay())));
myDiagram.allowDrop = true; // must be true to accept drops from the Palette
// initialize the Palette that is in a floating, draggable HTML container myPalette = new go.Palette("myPalette"); // must name or refer to the DIV HTML element myPalette.nodeTemplateMap = myDiagram.nodeTemplateMap; myPalette.model = new go.GraphLinksModel([ { text: "Lake", fill: grad1, stroke: brush1, figure: "Hexagon" }, { text: "Ocean", fill: grad2, stroke: brush2, figure: "Rectangle" }, { text: "Sand", fill: grad3, stroke: brush3, figure: "Diamond" }, { text: "Goldfish", fill: grad4, stroke: brush4, figure: "Octagon" } ]);
myPalette.addDiagramListener("InitialLayoutCompleted", function(diagramEvent) { var pdrag = document.getElementById("paletteDraggable"); var palette = diagramEvent.diagram; var paddingHorizontal = palette.padding.left + palette.padding.right; var paddingVertical = palette.padding.top + palette.padding.bottom; pdrag.style.width = palette.documentBounds.width + 20 + "px"; pdrag.style.height = palette.documentBounds.height + 30 + "px"; });
var info = document.getElementById("myInfo"); myDiagram.addChangedListener(function (e) { var sel = e.diagram.selection; var str = ""; if (sel.count === 0) { str = "Selecting nodes in the main Diagram will display information here."; info.innerHTML = str; return; } else if (sel.count > 1) { str = sel.count + " objects selected."; info.innerHTML = str; return; } // One object selected, display some information var elem = sel.first(); var shape = elem.findObject("SHAPE"); var txtblock = elem.findObject("TEXT"); str += "
Selected Node:
";
str += "
Figure: "
+ shape.figure + "
";
str += "
Text: "
+ txtblock.text + "
";
var strokeColor = shape.stroke; str += 'Color: '; info.innerHTML = str;
// Initialize color picker $("#custom").spectrum({ color: strokeColor,
// Change colors by constructing a gradient change: function(color) { var c = color.toRgb(); var r,g,b; var grad1 = new go.Brush(go.Brush.Linear); r = Math.min(c.r + 10, 255); g = Math.min(c.g + 10, 255); b = Math.min(c.b + 10, 255); grad1.addColorStop(0, "rgb(" + r +","+ g +","+ b + ")"); grad1.addColorStop(0.5, color.toRgbString()); r = Math.max(c.r - 30, 0); g = Math.max(c.g - 30, 0); b = Math.max(c.b - 30, 0); grad1.addColorStop(1, "rgb(" + r +","+ g +","+ b+ ")"); shape.fill = grad1; shape.stroke = "rgb(" + r +","+ g +","+ b+ ")";
txtblock.stroke = (r < 100 && g < 100 && b < 100) ? "white" : "black"; } }); });
$(function() { $("#paletteDraggable").draggable({handle: "#paletteDraggableHandle"}).resizable({ // After resizing, perform another layout to fit everything in the palette's viewport stop: function(){ myPalette.layoutDiagram(true); } }); $("#infoDraggable").draggable({handle: "#infoDraggableHandle"}); });
}