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| Jessie Wang =)
| | {{Template:JessieWang}} |
| | | <br> |
| {| border="1"
| | <center><font size=5>Welcome! =)</font></center> |
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| !Protein | |
| !Function
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| !Re-engineering
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| |-
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| |I
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| |Assembly
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| |Mutate the C terminus of the protein and see how that affects interactions with P4 and channel opening
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| |-
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| |II
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| |Replication of DNA + strand
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| |Mutate parts of the DNA - strand to see if P2 still nicks it. It would be interesting to find a particular motif to which it binds because P2 can then be used to nick other pieces of DNA.
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| |-
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| |III
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| |Phage tail protein (5 copies)
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| |Remove 3 copies of the gene and see how that affects M13
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| |-
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| |IV
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| |Assembly
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| |Alter the N terminus to make it interact more strongly with the C terminus of P1 and P11 and observe effects
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| |-
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| |V
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| |binds ssDNA
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| |Alter the gene so that it produces more/less of the protein to generate a curve of ssDNA vs dsDNA concentration.
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| |-
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| |VI
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| |Phage tail protein (5 copies)
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| |Make p6 protrude more and see if changes the behavior of M13
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| |-
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| |VII
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| |Phage head protein (5 copies)
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| |Withdraw P7 even more to observe changes, if any
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| |-
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| |VIII
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| |Phage coat proteins (2700 copies)
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| |Remove its ability to replace p5
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| |-
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| |IX
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| |Phage head protein (5 copies)
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| |Add more copies of p9
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| |-
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| |X
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| |DNA replication
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| |Increase production of p10 and see how that would also affect p2
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| |-
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| |XI
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| |Assembly
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| |Mutate the C terminus of the protein and see how that affects interactions with P4 and channel opening
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| |}
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