User:Jhdavis
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I recently joined the [[Sauer Lab]]. | I recently joined the [[Sauer Lab]]. | ||
| - | clpX holla!! | + | clpX holla!!!! |
| + | |||
| + | ==Protocols== | ||
| + | #Western Blots by Eyal [[Jhdavis::Western::Eyal]] | ||
| + | #GFP Purification by organic extraction [[Jhdavis::GFPOrganic]] | ||
| + | #Expression/Prep of ClpXdN-linked Trimers [[Jhdavis::ClpXPrep]] | ||
| + | #Peptide cleavage [[Jhdavis::PeptideCleavage]] | ||
| + | #Flp recombinase [[Jhdavis::Flping]] | ||
| + | #ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]] | ||
| + | #Sumo Protease expression/purification from Malakhov 2004 [[Jhdavis::SumoPrep]] | ||
| + | #regenerating qiagen columns [[Jhdavis::regenQiagen]] | ||
| + | #atpase notes [[Jhdavis::atpaseNotes]] | ||
| + | #Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) [[Jhdavis::Immunoprecip]] | ||
| + | |||
| + | ==Buffers== | ||
| + | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|PD Buffer]] | ||
| + | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | ||
| + | #[[Sauer:Making_yeg_plates|YEG-Cl]] | ||
| + | #[[Sauer:Davis Minimal Media]] | ||
| + | #[[Jhdavis::4x reducing SDS-PAGE loading buffer]] | ||
| + | |||
| + | ==Info== | ||
| + | *[[Jhdavis::GelFiltrationStandards]] | ||
| + | *[[Jhdavis::MetCleavage]] | ||
| + | *[[Jhdavis::lacIoperators]] | ||
| + | *[[Jhdavis::DNA 1 letter codes]] | ||
| + | *[[Jhdavis::Useful pymol]] | ||
| + | *[[Jhdavis::A260:A280 DNA:Protein ratios]] | ||
| + | *[[Jhdavis::fplc singup]] | ||
| + | |||
| + | ==Links== | ||
| + | #[[The_BioBricks_Foundation:Standards/Technical/E.coli_promoter_standard|Promoter standard]] | ||
| + | #[http://www.merckbiosciences.com/html/NVG/DuetTable.html novagen plasmid compatibility] | ||
| + | |||
| + | ==Sheets== | ||
| + | #[[Image:asdfsa.xlsx|Excel spreadsheet]] | ||
Current revision
I recently joined the Sauer Lab. clpX holla!!!!
Contents |
Protocols
- Western Blots by Eyal Jhdavis::Western::Eyal
- GFP Purification by organic extraction Jhdavis::GFPOrganic
- Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
- Peptide cleavage Jhdavis::PeptideCleavage
- Flp recombinase Jhdavis::Flping
- ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
- Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
- regenerating qiagen columns Jhdavis::regenQiagen
- atpase notes Jhdavis::atpaseNotes
- Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip
Buffers
- PD Buffer
- ATP Regeneration Mix
- YEG-Cl
- Sauer:Davis Minimal Media
- Jhdavis::4x reducing SDS-PAGE loading buffer
Info
- Jhdavis::GelFiltrationStandards
- Jhdavis::MetCleavage
- Jhdavis::lacIoperators
- Jhdavis::DNA 1 letter codes
- Jhdavis::Useful pymol
- Jhdavis::A260:A280 DNA:Protein ratios
- Jhdavis::fplc singup
