User:Jhdavis: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| (24 intermediate revisions by the same user not shown) | |||
| Line 5: | Line 5: | ||
#Western Blots by Eyal [[Jhdavis::Western::Eyal]] | #Western Blots by Eyal [[Jhdavis::Western::Eyal]] | ||
#GFP Purification by organic extraction [[Jhdavis::GFPOrganic]] | #GFP Purification by organic extraction [[Jhdavis::GFPOrganic]] | ||
#Expression/Prep of ClpXdN-linked Trimers [[Jhdavis::ClpXPrep]] | |||
#Peptide cleavage [[Jhdavis::PeptideCleavage]] | |||
#Flp recombinase [[Jhdavis::Flping]] | |||
#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]] | |||
#Sumo Protease expression/purification from Malakhov 2004 [[Jhdavis::SumoPrep]] | |||
#regenerating qiagen columns [[Jhdavis::regenQiagen]] | |||
#atpase notes [[Jhdavis::atpaseNotes]] | |||
#Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) [[Jhdavis::Immunoprecip]] | |||
==Buffers== | ==Buffers== | ||
#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|PD Buffer]] | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|PD Buffer]] | ||
#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | ||
#[[Sauer:Making_yeg_plates|YEG-Cl]] | |||
#[[Sauer:Davis Minimal Media]] | |||
#[[Jhdavis::4x reducing SDS-PAGE loading buffer]] | |||
==Info== | ==Info== | ||
*[[Jhdavis::GelFiltrationStandards]] | *[[Jhdavis::GelFiltrationStandards]] | ||
| Line 14: | Line 26: | ||
*[[Jhdavis::lacIoperators]] | *[[Jhdavis::lacIoperators]] | ||
*[[Jhdavis::DNA 1 letter codes]] | *[[Jhdavis::DNA 1 letter codes]] | ||
*[[Jhdavis::Useful pymol]] | |||
*[[Jhdavis::A260:A280 DNA:Protein ratios]] | |||
*[[Jhdavis::fplc singup]] | |||
==Links== | ==Links== | ||
#[[The_BioBricks_Foundation:Standards/Technical/E.coli_promoter_standard|Promoter standard]] | #[[The_BioBricks_Foundation:Standards/Technical/E.coli_promoter_standard|Promoter standard]] | ||
#[http://www.merckbiosciences.com/html/NVG/DuetTable.html novagen plasmid compatibility] | |||
==Sheets== | ==Sheets== | ||
[[Image: | #[[Image:asdfsa.xlsx|Excel spreadsheet]] | ||
Latest revision as of 11:26, 6 May 2009
I recently joined the Sauer Lab. clpX holla!!!!
Protocols
- Western Blots by Eyal Jhdavis::Western::Eyal
- GFP Purification by organic extraction Jhdavis::GFPOrganic
- Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
- Peptide cleavage Jhdavis::PeptideCleavage
- Flp recombinase Jhdavis::Flping
- ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
- Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
- regenerating qiagen columns Jhdavis::regenQiagen
- atpase notes Jhdavis::atpaseNotes
- Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip
Buffers
- PD Buffer
- ATP Regeneration Mix
- YEG-Cl
- Sauer:Davis Minimal Media
- Jhdavis::4x reducing SDS-PAGE loading buffer
Info
- Jhdavis::GelFiltrationStandards
- Jhdavis::MetCleavage
- Jhdavis::lacIoperators
- Jhdavis::DNA 1 letter codes
- Jhdavis::Useful pymol
- Jhdavis::A260:A280 DNA:Protein ratios
- Jhdavis::fplc singup
