User:Jhdavis: Difference between revisions
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#Flp recombinase [[Jhdavis::Flping]] | #Flp recombinase [[Jhdavis::Flping]] | ||
#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]] | #ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]] | ||
#Sumo Protease expression/purification from Malakhov 2004 [[ | #Sumo Protease expression/purification from Malakhov 2004 [[Jhdavis::SumoPrep]] | ||
#regenerating qiagen columns [[Jhdavis::regenQiagen]] | #regenerating qiagen columns [[Jhdavis::regenQiagen]] | ||
#atpase notes [[Jhdavis::atpaseNotes]] | |||
#Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) [[Jhdavis::Immunoprecip]] | |||
==Buffers== | ==Buffers== | ||
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#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | ||
#[[Sauer:Making_yeg_plates|YEG-Cl]] | #[[Sauer:Making_yeg_plates|YEG-Cl]] | ||
#[[Sauer:Davis Minimal Media]] | |||
#[[Jhdavis::4x reducing SDS-PAGE loading buffer]] | |||
==Info== | ==Info== | ||
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*[[Jhdavis::Useful pymol]] | *[[Jhdavis::Useful pymol]] | ||
*[[Jhdavis::A260:A280 DNA:Protein ratios]] | *[[Jhdavis::A260:A280 DNA:Protein ratios]] | ||
*[[Jhdavis::fplc singup]] | |||
==Links== | ==Links== | ||
Latest revision as of 11:26, 6 May 2009
I recently joined the Sauer Lab. clpX holla!!!!
Protocols
- Western Blots by Eyal Jhdavis::Western::Eyal
- GFP Purification by organic extraction Jhdavis::GFPOrganic
- Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
- Peptide cleavage Jhdavis::PeptideCleavage
- Flp recombinase Jhdavis::Flping
- ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
- Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
- regenerating qiagen columns Jhdavis::regenQiagen
- atpase notes Jhdavis::atpaseNotes
- Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip
Buffers
- PD Buffer
- ATP Regeneration Mix
- YEG-Cl
- Sauer:Davis Minimal Media
- Jhdavis::4x reducing SDS-PAGE loading buffer
Info
- Jhdavis::GelFiltrationStandards
- Jhdavis::MetCleavage
- Jhdavis::lacIoperators
- Jhdavis::DNA 1 letter codes
- Jhdavis::Useful pymol
- Jhdavis::A260:A280 DNA:Protein ratios
- Jhdavis::fplc singup
