User:Jhdavis

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Current revision (13:26, 6 May 2009) (view source)
(Buffers)
 
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I recently joined the [[Sauer Lab]].
I recently joined the [[Sauer Lab]].
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clpX holla!!!!
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==Protocols==
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#Western Blots by Eyal [[Jhdavis::Western::Eyal]]
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#GFP Purification by organic extraction [[Jhdavis::GFPOrganic]]
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#Expression/Prep of ClpXdN-linked Trimers [[Jhdavis::ClpXPrep]]
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#Peptide cleavage [[Jhdavis::PeptideCleavage]]
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#Flp recombinase [[Jhdavis::Flping]]
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#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]]
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#Sumo Protease expression/purification from Malakhov 2004 [[Jhdavis::SumoPrep]]
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#regenerating qiagen columns [[Jhdavis::regenQiagen]]
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#atpase notes [[Jhdavis::atpaseNotes]]
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#Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) [[Jhdavis::Immunoprecip]]
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==Buffers==
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#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|PD Buffer]]
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#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]]
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#[[Sauer:Making_yeg_plates|YEG-Cl]]
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#[[Sauer:Davis Minimal Media]]
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#[[Jhdavis::4x reducing SDS-PAGE loading buffer]]
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==Info==
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*[[Jhdavis::GelFiltrationStandards]]
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*[[Jhdavis::MetCleavage]]
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*[[Jhdavis::lacIoperators]]
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*[[Jhdavis::DNA 1 letter codes]]
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*[[Jhdavis::Useful pymol]]
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*[[Jhdavis::A260:A280 DNA:Protein ratios]]
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*[[Jhdavis::fplc singup]]
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==Links==
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#[[The_BioBricks_Foundation:Standards/Technical/E.coli_promoter_standard|Promoter standard]]
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#[http://www.merckbiosciences.com/html/NVG/DuetTable.html novagen plasmid compatibility]
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==Sheets==
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#[[Image:asdfsa.xlsx|Excel spreadsheet]]

Current revision

I recently joined the Sauer Lab. clpX holla!!!!

Contents

Protocols

  1. Western Blots by Eyal Jhdavis::Western::Eyal
  2. GFP Purification by organic extraction Jhdavis::GFPOrganic
  3. Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
  4. Peptide cleavage Jhdavis::PeptideCleavage
  5. Flp recombinase Jhdavis::Flping
  6. ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
  7. Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
  8. regenerating qiagen columns Jhdavis::regenQiagen
  9. atpase notes Jhdavis::atpaseNotes
  10. Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip

Buffers

  1. PD Buffer
  2. ATP Regeneration Mix
  3. YEG-Cl
  4. Sauer:Davis Minimal Media
  5. Jhdavis::4x reducing SDS-PAGE loading buffer

Info

Links

  1. Promoter standard
  2. novagen plasmid compatibility

Sheets

  1. Image:Asdfsa.xlsx
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