User:Jhdavis: Difference between revisions

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#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]]
#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]]
#Sumo Protease expression/purification from Malakhov 2004 [[Jhdaivs::SumoPrep]]
#Sumo Protease expression/purification from Malakhov 2004 [[Jhdaivs::SumoPrep]]
#regenerating qiagen columns [[Jhdavis::regenQiagen]]


==Buffers==
==Buffers==

Revision as of 07:14, 4 February 2009

I recently joined the Sauer Lab. clpX holla!!!!

Protocols

  1. Western Blots by Eyal Jhdavis::Western::Eyal
  2. GFP Purification by organic extraction Jhdavis::GFPOrganic
  3. Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
  4. Peptide cleavage Jhdavis::PeptideCleavage
  5. Flp recombinase Jhdavis::Flping
  6. ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
  7. Sumo Protease expression/purification from Malakhov 2004 Jhdaivs::SumoPrep
  8. regenerating qiagen columns Jhdavis::regenQiagen

Buffers

  1. PD Buffer
  2. ATP Regeneration Mix
  3. YEG-Cl

Info

Links

  1. Promoter standard
  2. novagen plasmid compatibility

Sheets

  1. File:Asdfsa.xlsx
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