User:Jlogan: Difference between revisions
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| II || | | II || modify such that it not only nicks the double stranded form of the genome to initiate replication of the + strand, but also nicks the - strand to impede the formation of dsDNA (this would also add p5) | ||
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| III || Add myc or other tag to monitor how it affects time delay/progression of phage escape from host | | III || Add myc or other tag to monitor how it affects time delay/progression of phage escape from host |
Revision as of 22:47, 12 February 2007
Name: Jennifer Logan
Email: jlogan at mit dot edu
M13 phage re-engineering ideas:
Gene | Ideas |
---|---|
I | |
II | modify such that it not only nicks the double stranded form of the genome to initiate replication of the + strand, but also nicks the - strand to impede the formation of dsDNA (this would also add p5) |
III | Add myc or other tag to monitor how it affects time delay/progression of phage escape from host |
IV | |
V | Modify such that it is able to sequester the positive stranded DNA more effectively, thus reducing the competition with dsDNA formation |
VI | As an accessory protein to p3, modify such that p3 is more effective in its interaction with TolA protein |
VII | Modify in such a way to allow for phage secretion speed to increase so the phage-host interaction time is decreased. |
VIII | Add myc or alternative tag to possibly aid in targeting various qualities of host |
IX | Same principle as gene VII except that care must be taken with this modification because it overlaps with gene 8. |
X | |
XI |