User:Jlogan
From OpenWetWare
Name: Jennifer Logan
Email: jlogan at mit dot edu
M13 phage re-engineering ideas:
Gene | Ideas |
---|---|
I | |
II | |
III | Add myc or other tag to monitor how it affects time delay/progression of phage escape from host |
IV | |
V | Modify such that it is able to sequester the positive stranded DNA more effectively, thus reducing the competition with dsDNA formation |
VI | As an accessory protein to p3, modify such that p3 is more effective in its interaction with TolA protein |
VII | Modify in such a way to allow for phage secretion speed to increase so the phage-host interaction time is decreased. |
VIII | Add myc or alternative tag to possibly aid in targeting various qualities of host |
IX | Same principle as gene VII except that care must be taken with this modification because it overlaps with gene 8. |
X | |
XI |