User:John Szymanski/notebook: Difference between revisions
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==Cell Cultures== | ==Cell Cultures== | ||
-LB solution with ampicillin | -LB solution with ampicillin <br> | ||
-sterile tubes with loose caps for air | -sterile tubes with loose caps for air <br> | ||
1) Label 2 tubes <br> | 1) Label 2 tubes <br> | ||
A) Control <br> | A) Control <br> | ||
| Line 16: | Line 16: | ||
5) Dip scraper in ethanol and flame briefly, then let cool<br> | 5) Dip scraper in ethanol and flame briefly, then let cool<br> | ||
6) Crack the lid of the plate, scrape off the colony, then immediately inoculate culture<br> | 6) Crack the lid of the plate, scrape off the colony, then immediately inoculate culture<br> | ||
7) | 7) incubate @ 37 C overnight with agitation<br><br> | ||
I made a culture for E.coli strain PRS305 | I made a culture for E.coli strain PRS305 | ||
Revision as of 14:28, 6 February 2008
Lab Notebook
Making Plates
Label plates with date, antibiotic present. Lay out all plates with lids on top. Lift lid, pour ~3mm of agar/LB/antibiotic solution, and immediately put lid back on.
Cell Cultures
-LB solution with ampicillin
-sterile tubes with loose caps for air
1) Label 2 tubes
A) Control
B) Strain of bacteria
2) Sterilize pipette
3) Pipette 3-10 mL of broth in each tube
4) Mark colonies to be cultured on plate
5) Dip scraper in ethanol and flame briefly, then let cool
6) Crack the lid of the plate, scrape off the colony, then immediately inoculate culture
7) incubate @ 37 C overnight with agitation
I made a culture for E.coli strain PRS305
