User:John Szymanski/notebook: Difference between revisions
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-LB solution with ampicillin <br> | -LB solution with ampicillin <br> | ||
-sterile tubes with loose caps for air <br> | -sterile tubes with loose caps for air <br> | ||
#Label 2 tubes: Control, Strain of bacteria<br> | |||
#Sterilize pipette<br> | |||
#Pipette 3-10 mL of broth in each tube<br> | |||
#Mark colonies to be cultured on plate<br> | |||
#Dip scraper in ethanol and flame briefly, then let cool<br> | |||
#Crack the lid of the plate, scrape off the colony, then immediately inoculate culture<br> | |||
#incubate @ 37 C overnight with agitation<br><br> | |||
I made a culture for E.coli strain PRS305<br><br> | I made a culture for E.coli strain PRS305<br><br> | ||
Revision as of 14:39, 6 February 2008
Lab Notebook
Making Plates
Label plates with date, antibiotic present. Lay out all plates with lids on top. Lift lid, pour ~3mm of agar/LB/antibiotic solution, and immediately put lid back on.
Cell Cultures
-LB solution with ampicillin
-sterile tubes with loose caps for air
- Label 2 tubes: Control, Strain of bacteria
- Sterilize pipette
- Pipette 3-10 mL of broth in each tube
- Mark colonies to be cultured on plate
- Dip scraper in ethanol and flame briefly, then let cool
- Crack the lid of the plate, scrape off the colony, then immediately inoculate culture
- incubate @ 37 C overnight with agitation
I made a culture for E.coli strain PRS305
