User:Jonathan Cline/Notebook/Melaminometer/Meetings: Difference between revisions
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| Line 90: | Line 90: | ||
* Discuss Schedule | * Discuss Schedule | ||
* Discuss current work | * Discuss current work | ||
** ELISA kit | ** ELISA kit study | ||
** | *** Assumed to be more costly than synbio solution, since kit needs purified enzymes in the kit | ||
** | *** Melaminometer must be better performance than ELISA kit to be successful | ||
*** Contradiction between biocyc.org and papers | ** Enzymatic method study | ||
** Antibody method | *** Contradiction between biocyc.org and papers and multiple action from triA | ||
*** Need to model this | *** Reproduce previous experimental result from 2001 paper; verify triA activity | ||
**** NYMU will request materials from author | |||
** Antibody method study | |||
*** Need to model this for melamine; find homologues | |||
*** 12227, 12228 from 2001-paper. | *** 12227, 12228 from 2001-paper. | ||
** Single plasmid should be possible | *** May be more successful method than pH detection | ||
** Single plasmid should be possible for the multiple genes; will make fabrication easier | |||
* Next steps | * Next steps | ||
## JC do patent search for related products | |||
## NYMU investigate ELISA kit | |||
## NYMU compare homologues of melamine deaminase | |||
Revision as of 19:45, 3 December 2008
Abbreviations:
- JC = jcline
- NYMU = National Yang Ming University, Taipei, Taiwan
- Chuan-Hsiung Chang's lab
- Chih-Hsien Yang OWW User:Blent
- MP = Meredith L. Patterson
2008-11-04 JC+NYMU
Attendees:
- JC
- NYMU
Conf call Summary
- Introductions
- Capabilities, existing NYMU technology & licensing
- product goal discussion
- OWW open source project
- set next meeting date/time 2008-11-18
2008-11-11 JC+SG
- Discussion of competitive methods for detection.
- ELISA
- You make a serial dilution and then get a good estimate of your antigen concentration by identifying the dilution at which you can first identify a color change.
2008-11-18 JC+NYMU
Attendees:
- JC
- NYMU
Conf call Summary
- postponed due to travel.
- set next meeting date/time 2008-11-25
2008-11-25 JC+NYMU
Attendees:
- JC
- NYMU
Conf call Summary
- Introductions
- NYMU team assignments
- Discuss NYMU presentation & JC OWW research
- Custom protein
- Example, combine light sensing protein in cyanobacteria with melamine receptor for detection
- Chassis
- Idea, use ADP1 as it has been proposed before
- Custom protein
- Set next steps:
- NYMU Want to get current market ELISA kit for evaluation & calibration in lab
- Need to scope where to get other materials
- NYMU Need to scope designing enzymes into e.coli
- JC to investigate creating biobricks from known seqs in research
- Need to investigate noise issues for detection in sample
- Need to investigate regulating enzymes
- Set next meeting date/time 2008-12-04 9AM (TAIPEI time)
JC Wiki Updates
- Created rough outline schedule on wiki
- created meeting notes on wiki
2008-12-4 JC+NYMU
Attendees:
- JC
- NYMU
- Chih-Hsien Yang
Conf call Summary
- Introductions
- Discuss team work method
- All data will be on wiki.
- No secondary mailing list or doc sharing site at this time. All docs uploaded to wiki.
- Discuss Schedule
- Discuss current work
- ELISA kit study
- Assumed to be more costly than synbio solution, since kit needs purified enzymes in the kit
- Melaminometer must be better performance than ELISA kit to be successful
- Enzymatic method study
- Contradiction between biocyc.org and papers and multiple action from triA
- Reproduce previous experimental result from 2001 paper; verify triA activity
- NYMU will request materials from author
- Antibody method study
- Need to model this for melamine; find homologues
- 12227, 12228 from 2001-paper.
- May be more successful method than pH detection
- Single plasmid should be possible for the multiple genes; will make fabrication easier
- ELISA kit study
- Next steps
- JC do patent search for related products
- NYMU investigate ELISA kit
- NYMU compare homologues of melamine deaminase
