User:Jonathan Cline/Notebook/Melaminometer/Proposed Research for Detection: Difference between revisions
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:: Omattage, the high school senior from Mississippi, said the weeklong event gave her a chance to meet other young scientists from around the world. | :: Omattage, the high school senior from Mississippi, said the weeklong event gave her a chance to meet other young scientists from around the world. | ||
:: another video, http://shelbinator.com/2008/05/16/and-georgia-tech-guys-complain/ | :: another video, http://shelbinator.com/2008/05/16/and-georgia-tech-guys-complain/ | ||
::: A recombinant M13 bacteriophage library was screened to identify peptide sequences with high affinity to cyanuric acid and melamine. Amino acid sequences STNFFYQTFAFH and RNSNHTAYGEEP were identified as the consensus sequences specific to cyanuric acid and melamine, respectively. Quartz crystal microbalance (QCM) based biosen-sors were developed using these peptides. Although cyanuric acid binding peptide demonstrated better mass accumulation rates than the melamine binding peptide, both peptides were capable of detecting their respective ligands at concentrations as low as 1.25 parts per billion (ppb) during the first five minutes of the experiment. Cyanuric acid and Melamine binding peptides coupled to a gold binding peptide sequence are currently being developed to increase the peptide density and sensitivity of the assay. Biosensors with synthetic peptides could decrease the detection limit to picomolar concentrations of the ligands. QCM instruments are portable, cost much less than GC-MS setups, and do not require highly trained personnel to operate. Therefore, QCM based biosensors may be used at ports and warehouses to more thoroughly screen food additives imported into the United States. In addition to food contaminants, the QCM based biosensors may also be employed to detect other harmful chemicals. | |||
Revision as of 19:16, 10 November 2008
New research into methods for detecting melamine.
- [YouTube Video] ISEF '08 Winner:Chemistry Bio-Sensors. Natalie Saranga Omattage, Intel International Science and Engineering Fair. Natalie Saranga Omattage of Cleveland MS, USA for the development of a more efficient and less expensive way to screen for potentially harmful food additive contaminants.
- Students from 51 countries competed in this year's International Science and Engineering Fair.
- Omattage, the high school senior from Mississippi, said the weeklong event gave her a chance to meet other young scientists from around the world.
- another video, http://shelbinator.com/2008/05/16/and-georgia-tech-guys-complain/
- A recombinant M13 bacteriophage library was screened to identify peptide sequences with high affinity to cyanuric acid and melamine. Amino acid sequences STNFFYQTFAFH and RNSNHTAYGEEP were identified as the consensus sequences specific to cyanuric acid and melamine, respectively. Quartz crystal microbalance (QCM) based biosen-sors were developed using these peptides. Although cyanuric acid binding peptide demonstrated better mass accumulation rates than the melamine binding peptide, both peptides were capable of detecting their respective ligands at concentrations as low as 1.25 parts per billion (ppb) during the first five minutes of the experiment. Cyanuric acid and Melamine binding peptides coupled to a gold binding peptide sequence are currently being developed to increase the peptide density and sensitivity of the assay. Biosensors with synthetic peptides could decrease the detection limit to picomolar concentrations of the ligands. QCM instruments are portable, cost much less than GC-MS setups, and do not require highly trained personnel to operate. Therefore, QCM based biosensors may be used at ports and warehouses to more thoroughly screen food additives imported into the United States. In addition to food contaminants, the QCM based biosensors may also be employed to detect other harmful chemicals.
