User:Jordan L. Metsky/Notebook/Phosphorylation/2012/01/27
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ObjectiveTo isolate t-butyl ammonium ATP. Description1. 1 L of a 50 mmol solution of butylammonium chloride was made.* 2. A column was elluted with 100 mL of the previously prepared 50x10^-3 solution of butylammonium chloride. Beads swelled for 20 minutes. 3. After 20 minutes, the column was elluted with 400 mL of the butylammonium chloride solution. Fractions were poured off. 4. 0.5 g of ATP was massed, and dissolved in about 100 mL of butylammonium chloride solution. The mixture was added to the column using a Pasteur pipette. 5. The column was elluted with an additional 300 mL of butylammonium chloride solution. 2 fractions were collected as follows: Fraction A was 150 mL-hypothetically containing the ATP solution and saved; Fraction B was 200 mL and poured off. 6. Fraction A was filtered using filter paper to remove solid matter (the mesh media from the column that had escaped into the solution), and transferred to a 500 mL flask. 7. The filtrate was distilled using a rotovap with H2O. 8. The filtrate obtained was a beige, opaque, oily residue.
Data1 L of 50 mmolar soln of tertbutylammonium chloride (C16H36ClN): (277.95 g/1 mol C16H36ClN) x (x g/50 x 10^/3 mmol) =13.896 g in 1 L H2O NotesThis area is for any observations or conclusions that you would like to note.
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