User:Jordan L. Metsky/Notebook/Phosphorylation/2012/02/03: Difference between revisions

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==Data==
==Data==
.4 g carbonyldimmidazole (CDI) was massed. 0.3 g [Ru(bpy)2 (phen-NH2)](PF6)2 taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/01/26|here]] was added to the CDI, and a vaccuum was applied, as well as nitrogen gas. Using a cannula and pressure from the nitrogen, the liquid [nBu<sub>4</sub>N]<sub>2</sub> ATP taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/02|here]] and ATP mixture was transferred to the CDI and [Ru(bpy)<sub>2</sub>(phen-NH2)](PF6)2 mixture. Nitrogen gas was applied, and the mixture was left to bubble overnight, as described in Lee, et al's "Synthesis amd reactivity of novel λ-phosphate modified ATP Analogs" 2009.
.4 g carbonyldimmidazole (CDI) was massed. 0.3 g [Ru(bpy)2 (phen-NH2)](PF6)2 taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/01/26|here]] was added to the CDI, and a vaccuum was applied to eliminate any water from the system, as well as nitrogen gas. Using a cannula, dry DMF was transferred to the Ru(bpy)2 (phen-NH2)](PF6)2 and CDI mixture. That mixture was cannula transferred to [nBu<sub>4</sub>N]<sub>2</sub> ATP taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/02|here]]. Nitrogen gas was applied. The resulting mixture was bright, clear orange in color, and contained solid [nBu<sub>4</sub>]<sub>2</sub> ATP. The mixture was left to bubble overnight on a stirplate, as described in Lee, et al's "Synthesis amd reactivity of novel λ-phosphate modified ATP Analogs" 2009.


==Notes==
==Notes==

Revision as of 11:30, 3 February 2012

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Objective

To create a [Ru(bpy)2(phen-NH2)](PF6)2 and carbonyldiimidazole solution.

This entry is a continuation from Dhea's Notebook.

Data

.4 g carbonyldimmidazole (CDI) was massed. 0.3 g [Ru(bpy)2 (phen-NH2)](PF6)2 taken from the procedure described here was added to the CDI, and a vaccuum was applied to eliminate any water from the system, as well as nitrogen gas. Using a cannula, dry DMF was transferred to the Ru(bpy)2 (phen-NH2)](PF6)2 and CDI mixture. That mixture was cannula transferred to [nBu4N]2 ATP taken from the procedure described here. Nitrogen gas was applied. The resulting mixture was bright, clear orange in color, and contained solid [nBu4]2 ATP. The mixture was left to bubble overnight on a stirplate, as described in Lee, et al's "Synthesis amd reactivity of novel λ-phosphate modified ATP Analogs" 2009.

Notes

This area is for any observations or conclusions that you would like to note.


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