User:Jordan L. Metsky/Notebook/Phosphorylation/2012/02/03: Difference between revisions
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To create a [Ru(bpy)2(phen-NH2)](PF6)2 and carbonyldiimidazole solution. | To create a [Ru(bpy)2(phen-NH2)](PF6)2 and carbonyldiimidazole solution. | ||
This entry is a continuation from Dhea's | This entry is a continuation from [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/02|Dhea's Notebook.]] | ||
==Data== | ==Data== | ||
.4 g carbonyldimmidazole (CDI) was massed. 0.3 g [Ru(bpy)2 (phen-NH2)](PF6)2 taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/01/26|here]] was added to the CDI, and a vaccuum was applied to eliminate any water from the system, as well as nitrogen gas. Using a cannula, dry DMF was transferred to the Ru(bpy)2 (phen-NH2)](PF6)2 and CDI mixture. That mixture was cannula transferred to [nBu<sub>4</sub>N]<sub>2</sub> ATP taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/02|here]]. Nitrogen gas was applied. The resulting mixture was bright, clear orange in color, and contained solid [nBu<sub>4</sub>]<sub>2</sub> ATP. The mixture was left to bubble overnight on a stir plate, as described in Lee, et al's "Synthesis and reactivity of novel λ-phosphate modified ATP Analogs" 2009. | |||
==Notes== | ==Notes== | ||
This work is continued [[User:Benjamin_D._Eachus/Notebook/Phosphorylation/2012/02/08|here]] | |||
[[Category:Course]] | [[Category:Course]] |
Revision as of 11:32, 10 February 2012
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ObjectiveTo create a [Ru(bpy)2(phen-NH2)](PF6)2 and carbonyldiimidazole solution. This entry is a continuation from Dhea's Notebook. Data.4 g carbonyldimmidazole (CDI) was massed. 0.3 g [Ru(bpy)2 (phen-NH2)](PF6)2 taken from the procedure described here was added to the CDI, and a vaccuum was applied to eliminate any water from the system, as well as nitrogen gas. Using a cannula, dry DMF was transferred to the Ru(bpy)2 (phen-NH2)](PF6)2 and CDI mixture. That mixture was cannula transferred to [nBu4N]2 ATP taken from the procedure described here. Nitrogen gas was applied. The resulting mixture was bright, clear orange in color, and contained solid [nBu4]2 ATP. The mixture was left to bubble overnight on a stir plate, as described in Lee, et al's "Synthesis and reactivity of novel λ-phosphate modified ATP Analogs" 2009. NotesThis work is continued here
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