User:Jordan L. Metsky/Notebook/Phosphorylation/2012/02/03: Difference between revisions

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To create a [Ru(bpy)2(phen-NH2)](PF6)2 and carbonyldiimidazole solution.
To create a [Ru(bpy)2(phen-NH2)](PF6)2 and carbonyldiimidazole solution.


This entry is a continuation from Dhea's notebook.
This entry is a continuation from [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/02|Dhea's Notebook.]]


==Data==
==Data==
* Add data and results here...
.4 g carbonyldimmidazole (CDI) was massed. 0.3 g [Ru(bpy)2 (phen-NH2)](PF6)2 taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/01/26|here]] was added to the CDI, and a vaccuum was applied to eliminate any water from the system, as well as nitrogen gas. Using a cannula, dry DMF was transferred to the Ru(bpy)2 (phen-NH2)](PF6)2 and CDI mixture. That mixture was cannula transferred to [nBu<sub>4</sub>N]<sub>2</sub> ATP taken from the procedure described [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/02|here]]. Nitrogen gas was applied. The resulting mixture was bright, clear orange in color, and contained solid [nBu<sub>4</sub>]<sub>2</sub> ATP.  The mixture was left to bubble overnight on a stir plate, as described in Lee, et al's "Synthesis and reactivity of novel λ-phosphate modified ATP Analogs" 2009.


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.




Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
This work is continued [[User:Benjamin_D._Eachus/Notebook/Phosphorylation/2012/02/08|here]]


[[Category:Course]]
[[Category:Course]]

Revision as of 11:32, 10 February 2012

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Objective

To create a [Ru(bpy)2(phen-NH2)](PF6)2 and carbonyldiimidazole solution.

This entry is a continuation from Dhea's Notebook.

Data

.4 g carbonyldimmidazole (CDI) was massed. 0.3 g [Ru(bpy)2 (phen-NH2)](PF6)2 taken from the procedure described here was added to the CDI, and a vaccuum was applied to eliminate any water from the system, as well as nitrogen gas. Using a cannula, dry DMF was transferred to the Ru(bpy)2 (phen-NH2)](PF6)2 and CDI mixture. That mixture was cannula transferred to [nBu4N]2 ATP taken from the procedure described here. Nitrogen gas was applied. The resulting mixture was bright, clear orange in color, and contained solid [nBu4]2 ATP. The mixture was left to bubble overnight on a stir plate, as described in Lee, et al's "Synthesis and reactivity of novel λ-phosphate modified ATP Analogs" 2009.

Notes

This work is continued here




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