User:Jordan L. Metsky/Notebook/Phosphorylation/2012/02/17: Difference between revisions

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==Description==
==Description==
Using the TEAB buffer created [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/16|here]] , a 0.1M in 2L H<sub>2</sub>O solution was created for use in the column.
Using the TEAB buffer created [[User:Dhea_Patel/Notebook/Phosphorylation/2012/02/16|here]] , a 0.1M in 2L H<sub>2</sub>O solution was created for use in the column.
ATP was dissolved in this buffer and eluted through the column. Five 50 mL fractions were collected. The fractions were a clear, and slightly orange in appearance. 
Glass plate chromatography was run on each of these fractions in a closed chamber, using ATP as a standard.
It was determined from chromatography that ATP was present only in the first fraction. This fraction was roto-vaped. Evaporation was slow, and many bubbles appeared.


==Data==
==Data==

Revision as of 14:49, 20 February 2012

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Objective

Learn how to maintain an OpenWetWare Notebook.

Description

Using the TEAB buffer created here , a 0.1M in 2L H2O solution was created for use in the column. ATP was dissolved in this buffer and eluted through the column. Five 50 mL fractions were collected. The fractions were a clear, and slightly orange in appearance.

Glass plate chromatography was run on each of these fractions in a closed chamber, using ATP as a standard.

It was determined from chromatography that ATP was present only in the first fraction. This fraction was roto-vaped. Evaporation was slow, and many bubbles appeared.

Data

  • Add data and results here...

Notes

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