User:Jordan L. Metsky/Notebook/Phosphorylation/2012/02/17

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Using the TEAB buffer created here , a 0.1M in 2L H2O solution was created for use in the column. ATP was dissolved in this buffer and eluted through the column. Five 50 mL fractions were collected. The fractions were a clear, and slightly orange in appearance.

Glass plate chromatography was run on each of these fractions in a closed chamber, using ATP as a standard.

It was determined from chromatography that ATP was present only in the first fraction. This fraction was roto-vaped. Evaporation was slow, and many bubbles appeared.


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