User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/08: Difference between revisions
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4. Run gel tp verify PCR and restrictions. | 4. Run gel tp verify PCR and restrictions. | ||
[[Image:|center| | [[Image:Gel_8Oct2010.JPG|center|180px]] | ||
'''Lanes:''' 1,9) Green ladder; 2) pSB1A2 + J23101 + ΔRBS + GFP E004 (Tube 1); 3) pSB1A2 + J23101 + ΔRBS + GFP E004 (Tube 2); 4) pSB1C3 (Preff FWD-Suff REV); 5) pSB3K3 + J23101 SpeI-PstI restriction; 6) pSB4A5 + MinBP SpeI-PstI restriction; 7) pSB1C3 EcoRI-PstI restriction; 8) ΔRBS + GFP E004 XbaI-PstI restriction. | '''Lanes:''' 1,9) Green ladder; 2) pSB1A2 + J23101 + ΔRBS + GFP E004 (Tube 1); 3) pSB1A2 + J23101 + ΔRBS + GFP E004 (Tube 2); 4) pSB1C3 (Preff FWD-Suff REV); 5) pSB3K3 + J23101 SpeI-PstI restriction; 6) pSB4A5 + MinBP SpeI-PstI restriction; 7) pSB1C3 EcoRI-PstI restriction; 8) ΔRBS + GFP E004 XbaI-PstI restriction. |
Revision as of 20:14, 8 October 2010
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October 8th, 20101. Make the following restrictions
Lanes: 1,9) Green ladder; 2) pSB1A2 + J23101 + ΔRBS + GFP E004 (Tube 1); 3) pSB1A2 + J23101 + ΔRBS + GFP E004 (Tube 2); 4) pSB1C3 (Preff FWD-Suff REV); 5) pSB3K3 + J23101 SpeI-PstI restriction; 6) pSB4A5 + MinBP SpeI-PstI restriction; 7) pSB1C3 EcoRI-PstI restriction; 8) ΔRBS + GFP E004 XbaI-PstI restriction.
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