User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/10: Difference between revisions
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== | ==October 10th, 2010== | ||
* | 1. Make XbaI-PstI double restriction to ΔRBS + GFP E004 Pure PCR product. | ||
*'''XbaI-PstI double restriction methods:''' | |||
:DNA -> 5 ul | |||
:Buffer 2 -> 2 ul (10% of total volume) | |||
:BSA -> 1 ul | |||
:XbaI -> 1 ul | |||
:PstI -> 1 ul | |||
:HPLC -> 10 ul (to complete total volume of 20ul) | |||
:Incubate at 37º C overnight | |||
Revision as of 17:56, 10 October 2010
 iGEM UNAM-Genomics-Mexico
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October 10th, 20101. Make XbaI-PstI double restriction to ΔRBS + GFP E004 Pure PCR product.
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