User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/20: Difference between revisions
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6. Make ligation: | 6. Inactivate restriction enzyme for 20 min at 80º C. | ||
7. Make ligation: | |||
*pSB1C3 (3ul) + [MinBP + ΔRBS + GFP E004] (5ul) | *pSB1C3 (3ul) + [MinBP + ΔRBS + GFP E004] (5ul) | ||
Revision as of 21:17, 20 October 2010
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October 20th, 20101. Run gel to verify results from PCR made on October 15th. Lanes: 1,12) Green ladder; 2-4) pSB4A5 + MinBP + ΔRBS + GFP E004 Colonies 1-3, 5-6) pSB4A5 + MinBP + ΔRBS + GFP E004 Colony 6 10; 7-10) pSB4A5 + MinBP + ΔRBS + GFP E004 Colonies 7-10; 11) BBa_I51020 amplified with Preff. FWD-Suff. REV.
into DH5-α competent cells, transform also BBa_I51020 (p19) (5 ul DNA) as control.
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