User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/20: Difference between revisions
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5. Transform the following ligations: | 5. Transform the following ligations: | ||
*pSB3K3 + J23101 | *pSB3K3 + J23101 with ΔRBS + GFP E004 (20ul) | ||
into DH5-α competent cells, transform also BBa_I51020 (p19) (5 ul DNA) as control. | into DH5-α competent cells, transform also BBa_I51020 (p19) (5 ul DNA) as control. | ||
*'''Transformation method:''' | *'''Transformation method:''' |
Revision as of 12:34, 21 October 2010
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October 20th, 20101. Run gel to verify results from PCR made on October 15th. Lanes: 1,12) Green ladder; 2-4) pSB4A5 + MinBP + ΔRBS + GFP E004 Colonies 1-3, 5-6) pSB4A5 + MinBP + ΔRBS + GFP E004 Colony 6 10; 7-10) pSB4A5 + MinBP + ΔRBS + GFP E004 Colonies 7-10; 11) BBa_I51020 amplified with Preff. FWD-Suff. REV.
into DH5-α competent cells, transform also BBa_I51020 (p19) (5 ul DNA) as control.
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