User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/09: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2011/05/09 Entry for User:Jose_Fabricio_Lopez/Notebook/Logbook)
 
(3 intermediate revisions by the same user not shown)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==WetLab==
* Insert content here...
===Plasmid extraction pBBRmcS5. From DH5α.===
**First lane control
**Plasmids 7 lanes.
 
[[Image:9Mayo2011.pBBRmcS5.Extracción..JPG]]
 
===Plasmid digestion===
*7 digestions with HindIII
**2μL 10X Buffer 2 NE
**8μL H<sub>2</sub>0
**10μL DNA Plasmid stock
**1μL Hind III.
*Marks,
**1 tube without DNA
**1 tube without HindIII
**7 tubes with each plasmid stock.




<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
 


__NOTOC__
__NOTOC__

Revision as of 11:45, 1 July 2011

iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

WetLab

Plasmid extraction pBBRmcS5. From DH5α.

    • First lane control
    • Plasmids 7 lanes.

Plasmid digestion

  • 7 digestions with HindIII
    • 2μL 10X Buffer 2 NE
    • 8μL H20
    • 10μL DNA Plasmid stock
    • 1μL Hind III.
  • Marks,
    • 1 tube without DNA
    • 1 tube without HindIII
    • 7 tubes with each plasmid stock.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Jose_Fabricio_Lopez/Notebook/Logbook/2011/05/09&oldid=520458"